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Abstract
Pharmaceutical Care and Research: 2018; 18(3):195-198
DOI: 10.5428/pcar20180309
Study on the quality standards of Bailongjieyu granules
1. LIN HongWei(Department of Pharmacy,Changzheng Hospital,Second Military Medical University,Shanghai 200003,China lhwayxxsh@163.com)
2. JIANG ChunXia(Department of Pharmacy,Changzheng Hospital,Second Military Medical University,Shanghai 200003,China )
3. PIAO ShuJuan(Department of Pharmacy,Changzheng Hospital,Second Military Medical University,Shanghai 200003,China )
4. LI JingXian(Department of Pharmacy,Changzheng Hospital,Second Military Medical University,Shanghai 200003,China )
5. LU WenQuan(Department of Pharmacy,Changzheng Hospital,Second Military Medical University,Shanghai 200003,China lwqp@163.com)
ABSTRACT  Objective: To establish a new method for the quality control of Bailongjieyu granules,so as to improve its quality standards.Methods: Bupleurum,Paeoniae Radix Alba,Bupleurum chinense DC. and Angelica sinensis were identified by TLC method. Content of peoniflorin in Bailongjieyu granules was determined by HPLC method.Agilent Zorbax SB-C18 column (250 mm×4.6 mm,5 μm) was employed with the mobile phase consisting of acetonitrile(phase A)-0.1% phosphoric acid solution(phase B) in gradient elution(0-49 min 88%-70% phase B,50-51 min 70%-5% phase B,52-59 min 5% phase B,60-61 min 5%-88% phase B,62-70 min 88% phase B)at a flow rate of 1 ml/min.The detection wavelength was 230 nm,the column temperature was 25 ℃,and the sample size was 10 μl.Results: TLC identification of Bupleurum,Paeoniae Radix Alba,Bupleurum chinense DC. and Angelica sinensis was specific, with clear color display.The precision,repeatability and stability of paeoniflorin conformed to the standards of Pharmacopoeia of the People’s Republic of China (2015 ed).Peoniflorin displayed good linearity within the ranges of 12.001-120.01 μg/ml (r=0.999 9).The recovery rate was (99.45±0.81)%(n=9).Conclusion: The established TLC and HPLC methods could be used for the quality control of Bailongjieyu granules.
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Please cite this article as:
LIN HongWei,JIANG ChunXia,PIAO ShuJuan,LI JingXian,LU WenQuan,. Study on the quality standards of Bailongjieyu granules[J]. Pharmaceutical Care and Research / yao xue fu wu yu yan jiu. 2018; 18(3): 195-198.
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