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Abstract
Pharmaceutical Care and Research: 2018; 18(4):304-307
DOI: 10.5428/pcar20180419
Determination of nucleosides contents in Cordyceps sinensis from different origins and its counterfeit products
1. FENG Xu(Naval Medical Research Institute,Second Military Medical University,Shanghai 200433,China zhenlunfeng@163.com)
2. WANG JiaChun(Naval Medical Research Institute,Second Military Medical University,Shanghai 200433,China )
3. ZOU YingXin(Naval Medical Research Institute,Second Military Medical University,Shanghai 200433,China )
4. CHU ZhiYong(Naval Medical Research Institute,Second Military Medical University,Shanghai 200433,China )
5. ZHANG ZhenZhen(Naval Medical Research Institute,Second Military Medical University,Shanghai 200433,China zz_jane@163.com)
ABSTRACT  Objective: To establish a HPLC method for the determination of three kinds of nucleosides(uridine,guanosine and adenosine),so as to analyze the contents of the three nucleosides in authentic Cordyceps sinensis,its counterfeit products and subtitutes on the market.Methods: Chromatography separation was performed on the Zorbax Eclipse Plus C18 column (250 mm×4.6 mm,5 μm) with methanol-water as mobile phase in a gradient elution(0-10 min 5% methanol,11-15 min 7% methanol,16-20 min 15% methanol,21-25 min 30% methanol).Detection wavelength was 260 nm, flow rate was 1.0 ml/min, column temperature was 30 ℃, and injection volume was 20 μl.Results: The concentrations and the peak areas of the three kinds of nucleosides within the range of 1-25 μg/ml was in good linearity(r=0.999 9).The methodological indicators of presicion,stability and recovery rate all satisty the required demands.The average contents of uridine and guanosine in authentic C. sinensis were higher than those in the counterfeit produces and substitutes.Conclusion: The contents of uridine and guanosine in authentic C. sinensis are obviously higher than those in the counterfeit products and substitutes.It may provide reference for the identification of authentic C. sinensis.
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Please cite this article as:
FENG Xu,WANG JiaChun,ZOU YingXin,CHU ZhiYong,ZHANG ZhenZhen,. Determination of nucleosides contents in Cordyceps sinensis from different origins and its counterfeit products[J]. Pharmaceutical Care and Research / yao xue fu wu yu yan jiu. 2018; 18(4): 304-307.
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