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Abstract
Pharmaceutical Care and Research: 2018; 18(5):372-376
DOI: DOI:10.5428/pcar20180514
Rapid determination of rat plasma concentration of sufentanil by thin layer chromatography-surface enhanced Raman spectroscopy method
1. ZHANG Tian1(1.Teaching and Research Section of Pharmaceutical Analysis,School of Pharmacy,Second Military Medical University,Shanghai 200433,China 505452521@qq.com)
2. ZHU QingXia1(1.Teaching and Research Section of Pharmaceutical Analysis,School of Pharmacy,Second Military Medical University,Shanghai 200433,China )
3. 2(2.Department of Pharmacy,Shanghai Ninth People’s Hospital Affiliated to School of Medicine,Shanghai Jiaotong University,Shanghai 200011,China )
4. WU ZeBing1(1.Teaching and Research Section of Pharmaceutical Analysis,School of Pharmacy,Second Military Medical University,Shanghai 200433,China )
5. 2(2.Department of Pharmacy,Shanghai Ninth People’s Hospital Affiliated to School of Medicine,Shanghai Jiaotong University,Shanghai 200011,China )
6. LU Feng1(1.Teaching and Research Section of Pharmaceutical Analysis,School of Pharmacy,Second Military Medical University,Shanghai 200433,China feng lufeng@hotmail.com)
7. QI YunPeng1(1.Teaching and Research Section of Pharmaceutical Analysis,School of Pharmacy,Second Military Medical University,Shanghai 200433,China qiyunpeng@smmu.edu.cn)
ABSTRACT  Objective: To establish a method for rapid determination of sufentanil concentration in rat plasma by thin layer chromatography-surface enhanced Raman spectroscopy(TLC-SERS) method.Methods: The plasma samples were separated by TLC with dichloromethane-methanol(9∶1.2) as a developing agent,and then SERS was applied for qualitative and quantitative analysis of sufentanil.Linear regression was performed between the concentration of sufentanil and the intensity of the Raman characteristic peak at 1004 cm-1.The standard curve,detection limit and recovery rate were all investigated.Results: The concentration of sufentanil within the range of 0.85-85.00 μg/ml and the peak intensity of SERS at 1004 cm-1 were in good linearity.The detection limit was 0.85 μg/ml,and the recovery rates of low (425 μg/ml) and high concentrations (4250 μg/ml ) were (86.00±8.15)% and (103.45±3.59)%(n=3),respectively.Conclusion: The study established a simple,rapid and practical method for the determination of sufentanil plasma concentration.
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Please cite this article as:
ZHANG Tian1,ZHU QingXia1,2,WU ZeBing1,2,LU Feng1,QI YunPeng1,. Rapid determination of rat plasma concentration of sufentanil by thin layer chromatography-surface enhanced Raman spectroscopy method[J]. Pharmaceutical Care and Research / yao xue fu wu yu yan jiu. 2018; 18(5): 372-376.
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