Your Navigation:PCAR > Abstract
Search pcar
检索项:
检索词:
Abstract
Pharmaceutical Care and Research: 2018; 18(6):424-428
DOI: 10.5428/pcar20180608
Preparation and in vitro evaluation of a polypeptide nanocomposite pPMEPA1 of anti-prostate cancer cell migration
1. LU Yue1(1.Teaching and Research Section of Pharmaceutics,School of Pharmacy,East China University of Science and Technology,Shanghai 200237,China lory0310@163.com)
2. DENG ZhaiZhu1(1.Teaching and Research Section of Pharmaceutics,School of Pharmacy,East China University of Science and Technology,Shanghai 200237,China )
3. DAI Qi1(1.Teaching and Research Section of Pharmaceutics,School of Pharmacy,East China University of Science and Technology,Shanghai 200237,China )
4. WU Xin2(2.Shanghai Weier Lab,Shanghai 200433,China )
5. REN FuZheng1(1.Teaching and Research Section of Pharmaceutics,School of Pharmacy,East China University of Science and Technology,Shanghai 200237,China fzren@ecust.edu.cn)
ABSTRACT  Objective: To prepare a disulfide-bonded arginine-aspartic acid polypeptide polymer(SRD) as a gene delivery carrier of plasmid PMEPA1(pPMEPA1,DPM) and to evaluate its anti-prostate cancer cell migration ability in vitro.Methods: SRD was synthesized by using H2O2 as oxidant and cysteine as cross linking agent.The structure of SRD was characterized by 1H-NMR.The SRD/DPM compounds were prepared by mixing SRD and DPM at various nitrogen/phosphorus(N/P) ratios.Zeta sizer was used to measure the particle size and zeta potential of SRD/DPM compounds,and agarose gel electrophoresis(AGE) was employed to determine the DPM condensation ability of SRD.Cytotoxicity of the carriers was measured through incubating SRD and SRD/DPM compounds with prostate cancer cell DU145.Cellular uptake efficiency of SRD/DPM compounds was measured by a flow cytometer using YOYO-1 labeled DPM as an indicator.Transwell assay was performed to determine cell migration ability of SRD/DPM compounds to DU145 cells in vitro.Results: 1H-NMR spectrum showed that the gene carrier SRD was successfully synthesized.SRD and DPM were combined to form a nanocomposite by electrostatic attraction,and the surface was positively charged.AGE results showed that SRD with N/P>2.5 had strong loading capacity to gene drugs.After incubating SRD with DU145 cells for 24 h,the cell viability was still more than 50%.Cell uptake and Transwell experiment indicated that SRD could significantly enhance the uptake of DPM by DU145 cells and improve the effects of DPM on the migration of DU145 cells when it was compared with RD monomer.Conclusion: Delivery of DPM with SRD as a gene drug carrier could inhibit prostate cancer cell migration and was expected to be a promising treatment of prostate cancer bone metastasis.
Welcome to PCAR! You are the number 264 reader of this article!
Please cite this article as:
LU Yue1,DENG ZhaiZhu1,DAI Qi1,WU Xin2,REN FuZheng1,. Preparation and in vitro evaluation of a polypeptide nanocomposite pPMEPA1 of anti-prostate cancer cell migration[J]. Pharmaceutical Care and Research / yao xue fu wu yu yan jiu. 2018; 18(6): 424-428.
References:
1. LIAO Qi,HUANG Yi,XU Xue.Pain management of bone metastasis in prostate cancer[J].Med Inf,2018,31(6):51-54.In Chinese with English abstract.
2. Ginn S L,Amaya A K,Alexander I E,et al.Gene therapy clinical trials worldwide to 2017:an update[J].J Gene Med,2018,20(5):e3015.
3. Fournier P G J,Juárez P,JIANG GuangLong,et al.The TGF-β signaling regulator PMEPA1 suppresses prostate cancer metastases to bone[J].Cancer Cell,2015,27(6):809-821.
4. GUO ZhengRong,PENG HuanYan,KANG JiWen,et al.Cell-penetrating peptide-a novel non-viral vector[J].China Biotechnol,2013,36(6):100-106.In Chinese.
5. LIU HongMei,WANG Hui,YANG WenJun,et al.Disulfide cross-linked low generation dendrimers with high gene transfection efficacy,low cytotoxicity,and low cost[J].J Am Chem Soc,2012,134(42):17680-17687.
6. Deshantri A K,Moreira A V,Ecker V,et al.Nanomedicines for the treatment of hematological malignancies[J].J Contr Release,2018,287(1):194-215.
7. WEI RongRan,CHENG Liang,ZHENG Meng,et al.Reduction-responsive disassemblable core-cross-linked micelles based on poly(ethylene glycol)-b-poly(N-2-hydroxypropyl methacrylamide)-lipoic acid conjugates for triggered intracellular anticancer drug release[J].Biomacromolecules,2012,13(8):2429-2438.
8. CHENG Ru,FENG Fang,MENG FengHua,et al.Glutathione-responsive nano-vehicles as a promising platform for targeted intracellular drug and gene delivery[J].J Contr Release,2011,152(1):2-12.
《药学服务与研究》杂志社 All Rights Reserved 网站备案号:鲁B2-20061008
·地址:上海市杨浦区长海路168号18号楼东三楼 邮政编码:200433
·联系电话(传真):86-21-65519829, 021-31162330
·电子邮件:PharmCR@vip.163.com
·技术支持:中国康网
管理员入口