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Abstract
Pharmaceutical Care and Research: 2018; 18(6):444-447
DOI: 10.5428/pcar20180612
Content determination of triptolide in triptolide nanoemulsion by LC/MS method
1. FAN ZhaoFeng(Department of Pharmacy,Renji Hospital Affiliated to School of Medicine,Shanghai Jiaotong University,Shanghai 200127,China 13818149898@163.com)
2. GUI XiaoLong(Department of Pharmacy,Renji Hospital Affiliated to School of Medicine,Shanghai Jiaotong University,Shanghai 200127,China )
3. HUANG SaiJie(Department of Pharmacy,Renji Hospital Affiliated to School of Medicine,Shanghai Jiaotong University,Shanghai 200127,China )
ABSTRACT  Objective: To establish a LC/MS method for content determination of triptolide in triptolide nanoemulsion.Methods: ZORBAX Extend-C18 column (100 mm×2.1 mm,3.5 μm) was used with a mobile phase of 0.1% (V/V) formic acid aqueous solution∶methanol(40∶60) at a flow rate of 0.3 ml/min.Column temperature was 40 ℃ and injection volume was 30 μl.Mass spectrometry was performed using multiple reaction monitoring(MRM) electrospray ionization(ESI) combined with positive ion scanning switch.The influence of the excimer ions,fragment ions,dry gas flow rate,nebulizer pressure,cracking voltage and collision energy on content determination of triptolide was evaluated.Content determination methodology of TPL was investigated after mass spectrometry conditions were confirmed.Results: The mass spectrometry methods were as follows:quantitative analysis ions were m/z=361.3/105.2,drying gas flow rate was 8 L/min,drying gas temperature was 350 ℃,capillary voltage was 4000 V,nebulizer pressure was 275.79 kPa(40 psi),cracker voltage was 105 eV and collision energy was 33.The content determination method showed good specificity,and triptolide displayed good linearity within the range of 10-500 ng/ml (r=0.999 9).The sample was basically stable within 8 hours after treatment,and inter- and intra-day densities were less than 5.55%.The average recovery rate was (99.90±1.32)%(n=6).The content of triptolide in triptolide nanoemulsion was (242.9±2.10) μg/g(n=3).Conclusion: The established LC/MS method seemed to be accurate,sensitive and reproducible,and could be used for content determination of the drug in triptolide nanoemulsion.
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Please cite this article as:
FAN ZhaoFeng,GUI XiaoLong,HUANG SaiJie,. Content determination of triptolide in triptolide nanoemulsion by LC/MS method[J]. Pharmaceutical Care and Research / yao xue fu wu yu yan jiu. 2018; 18(6): 444-447.
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